What Is Reconstitution?

Reconstitution transforms a peptide from its stable, freeze-dried powder form into a liquid solution suitable for precise measurement and administration. The process requires sterile technique: bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is drawn into a syringe and injected slowly down the inside wall of the peptide vial, allowing the powder to dissolve without agitation. Swirling gently is acceptable, but shaking can denature the peptide by disrupting its molecular structure. The resulting concentration depends on the amount of water added; for example, adding 2 mL of water to a 5 mg vial creates a 2.5 mg/mL concentration. Reconstituted peptides must be refrigerated at 2-8°C and typically remain stable for 3-4 weeks.